Vitamin D Total Test – 25-Hydroxy Vitamin D

The Vitamin D Total Test is a blood test that measures the combined concentration of two major circulating forms of vitamin D: 25-Hydroxy Vitamin D2 and 25-Hydroxy Vitamin D3.

Vitamin D plays an important role in calcium and phosphorus absorption, bone development, muscle function, and maintaining healthy bones.

The total 25-OH Vitamin D measurement is commonly used to assess vitamin D status.

What Does the Vitamin D Total Test Measure?

• 25-OH Vitamin D2: Measures the circulating form derived mainly from dietary sources and certain supplements.
• 25-OH Vitamin D3: Measures the circulating form produced in the skin through sunlight exposure and obtained from dietary sources.
• Total 25-OH Vitamin D: Represents the combined D2 and D3 concentration.

Why Is the Vitamin D Total Test Recommended?

• Vitamin D Deficiency: Helps identify low vitamin D levels.
• Bone Health: Supports evaluation of bone-related conditions.
• Calcium Metabolism: Helps assess vitamin D’s role in calcium absorption.
• Muscle Function: Supports investigation of selected muscle-related symptoms.
• Treatment Monitoring: Helps monitor vitamin D supplementation when clinically indicated.

Sample Requirements

• Sample type: Blood, typically serum.
• Fasting: Usually not required.
• Preparation: Inform your healthcare professional about vitamin D supplements and medicines.

Understanding Your Results

• Low levels: May indicate vitamin D deficiency or insufficiency.
• Adequate levels: Suggest sufficient vitamin D status according to the laboratory’s reference range.
• High levels: May indicate excessive supplementation and potential vitamin D toxicity.

Results should be interpreted according to the laboratory’s validated reference intervals and the patient’s clinical condition.

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